Electroacupuncture (EA) treatment has been widely used for stroke-like disorders in traditional Chinese medicine. However, the\nunderlying mechanisms remain unclear. Our previous studies showed that single-time EA stimulation at ââ?¬Å?Baihuiââ?¬Â (GV 20) and\nââ?¬Å?Shuigouââ?¬Â (GV 26) after the onset of ischemia can protect the brain against ischemic injury in rats with middle cerebral artery\nocclusion (MCAO). Here, we further investigated the differential effects between multiple EA and single-time EA stimulation on\nischemic injury. In the present study, we found that both single-time EA andmultiple EA stimulation significantly reducedMCAOinduced\nischemic infarction, while only multiple EA attenuated sensorimotor dysfunctions. Also, with PCR array screening and\ningenuity gene analysis,we revealed thatmultiple EA and single-time EA stimulation could differentially induce expression changes\nin neurotrophic signaling related genes.Meanwhile,withwestern blotting,we demonstrated that the level of glia maturation factor????\n(GMF????) increased in the early stage (day 1) of reperfusion, and this upregulation was suppressed only by single-time EA stimulation.\nThese findings suggest that the short-term effect of single-time EA stimulation differs from the cumulative effect of multiple EA,\nwhich possibly depends on their differential modulation on neurotrophic signaling molecules expression.
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